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Biology - Size of Specimens

Grade 9IGCSE

Review the key concepts, formulae, and examples before starting your quiz.

🔑Concepts

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Magnification (MM) is defined as the ratio of the size of an image (II) to the actual size (AA) of the specimen being observed.

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The SI units commonly used for specimen size in biology are millimeters (mmmm), micrometers (μm\mu m), and nanometers (nmnm).

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Conversion factors are essential: 1mm=1000μm1 mm = 1000 \mu m and 1μm=1000nm1 \mu m = 1000 nm. Therefore, 1mm=1,000,000nm1 mm = 1,000,000 nm.

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To calculate magnification from a drawing, use a ruler to measure the size of the drawing (Image size) and compare it to the given actual size of the organism.

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A scale bar is a line drawn next to a specimen that represents a specific actual length. To find the magnification of a micrograph, measure the length of the scale bar with a ruler and divide it by the value indicated on the bar.

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When performing calculations, always ensure that both the Image size (II) and the Actual size (AA) are converted to the same units (usually mmmm or μm\mu m) before dividing.

📐Formulae

M=IAM = \frac{I}{A}

A=IMA = \frac{I}{M}

I=M×AI = M \times A

Value in μm=Value in mm×1000\text{Value in } \mu m = \text{Value in } mm \times 1000

💡Examples

Problem 1:

A student measures a drawing of a bacterial cell to be 45mm45 mm long. The actual length of the bacterium is 3μm3 \mu m. Calculate the magnification of the drawing.

Solution:

First, convert the Image size (II) to micrometers: 45mm×1000=45000μm45 mm \times 1000 = 45000 \mu m Next, apply the magnification formula: M=IAM = \frac{I}{A} M=45000μm3μmM = \frac{45000 \mu m}{3 \mu m} M=15000×M = 15000 \times

Explanation:

To calculate magnification, units must be consistent. Converting 45mm45 mm to 45000μm45000 \mu m allows us to divide by the actual size of 3μm3 \mu m, resulting in a magnification of 1500015000 times the original size.

Problem 2:

A micrograph of a leaf cross-section has a magnification of 250×250 \times. If the thickness of the leaf in the image is 3.5cm3.5 cm, what is the actual thickness of the leaf in micrometers (μm\mu m)?

Solution:

First, convert the Image size (II) from cmcm to mmmm: 3.5cm=35mm3.5 cm = 35 mm Now, find the Actual size (AA) in mmmm: A=IMA = \frac{I}{M} A=35mm250=0.14mmA = \frac{35 mm}{250} = 0.14 mm Finally, convert the actual size to micrometers: 0.14mm×1000=140μm0.14 mm \times 1000 = 140 \mu m

Explanation:

The image size is measured as 3.5cm3.5 cm, which is 35mm35 mm. Dividing by the magnification (250250) gives the actual size in mmmm (0.14mm0.14 mm). Multiplying by 10001000 converts this to the standard biological unit of micrometers.

Problem 3:

A scale bar on an electron micrograph is 20mm20 mm long and is labeled as representing 500nm500 nm. Calculate the magnification of the micrograph.

Solution:

Convert the scale bar length (II) to nmnm: 20mm×1000=20000μm20 mm \times 1000 = 20000 \mu m 20000μm×1000=20,000,000nm20000 \mu m \times 1000 = 20,000,000 nm Apply the formula: M=20,000,000nm500nmM = \frac{20,000,000 nm}{500 nm} M=40000×M = 40000 \times

Explanation:

The scale bar acts as the 'Image' of the 'Actual' value it labels. By converting 20mm20 mm into 20,000,000nm20,000,000 nm, we can divide by the labeled 500nm500 nm to find that the image has been magnified 40,00040,000 times.