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Biology - Biological molecules (Carbohydrates, Lipids, Proteins, and DNA tests)

Grade 9IGCSE

Review the key concepts, formulae, and examples before starting your quiz.

🔑Concepts

Biological molecules are composed primarily of Carbon (CC), Hydrogen (HH), and Oxygen (OO). Proteins also contain Nitrogen (NN) and sometimes Sulfur (SS). Nucleic acids like DNA contain Phosphorus (PP).

Carbohydrates are used for energy. Monosaccharides like glucose (C6H12O6C_6H_{12}O_6) are simple sugars. Polysaccharides include starch (energy storage in plants), glycogen (energy storage in animals), and cellulose (cell wall structure).

Lipids (fats and oils) are made from one molecule of glycerol and three molecules of fatty acids. they are used for long-term energy storage, insulation, and cell membranes.

Proteins are polymers made of long chains of amino acids. The sequence of amino acids determines the 3D3D shape and specific function of the protein, such as enzymes, antibodies, or hemoglobin.

DNA (Deoxyribonucleic acid) is a double helix made of two strands of nucleotides. The strands are held together by complementary base pairs: Adenine (AA) pairs with Thymine (TT), and Cytosine (CC) pairs with Guanine (GG).

Food Test for Starch: Add Iodine solution. A positive result is a color change from orange-brown to blue-black.

Food Test for Reducing Sugars: Add Benedict's reagent and heat in a water bath at 80C80^{\circ}C. A positive result changes from blue to green, yellow, orange, or brick-red (depending on concentration).

Food Test for Proteins: Add Biuret reagent (Sodium Hydroxide and Copper (II) Sulfate). A positive result is a color change from blue to violet/purple.

Food Test for Lipids: Perform the Ethanol emulsion test. Dissolve the sample in Ethanol, then add to water. A positive result is the formation of a cloudy white emulsion.

📐Formulae

C6H12O6 (Glucose)C_6H_{12}O_6 \text{ (Glucose)}

Cn(H2O)n (General formula for Monosaccharides)C_n(H_2O)_n \text{ (General formula for Monosaccharides)}

Glycerol+3×Fatty AcidsTriglyceride+3H2O\text{Glycerol} + 3 \times \text{Fatty Acids} \rightarrow \text{Triglyceride} + 3H_2O

Amino Acid1+Amino Acid2Dipeptide+H2O\text{Amino Acid}_1 + \text{Amino Acid}_2 \rightarrow \text{Dipeptide} + H_2O

💡Examples

Problem 1:

A student tests a mystery food sample. The sample gives a blue result with Benedict's reagent after heating, but turns blue-black when Iodine solution is added. What can be concluded about the sample?

Solution:

The sample contains starch but does not contain reducing sugars.

Explanation:

Iodine solution turning blue-black is the specific indicator for the presence of starch. Benedict's reagent remaining blue (its original color) indicates that no reducing sugars, such as glucose or maltose, are present in detectable amounts.

Problem 2:

State the complementary base sequence for a DNA strand with the sequence: AGCTTAA-G-C-T-T-A.

Solution:

TCGAATT-C-G-A-A-T

Explanation:

In DNA, base pairing is specific due to the molecular structure: Adenine (AA) always pairs with Thymine (TT), and Cytosine (CC) always pairs with Guanine (GG).

Problem 3:

Which elements are found in a molecule of insulin (a protein) that are not found in a molecule of glycogen (a polysaccharide)?

Solution:

Nitrogen (NN) and often Sulfur (SS).

Explanation:

Carbohydrates like glycogen only contain CC, HH, and OO. Proteins are made of amino acids, which always contain a Nitrogen-based amine group (NH2-NH_2), and some amino acids (like cysteine) contain Sulfur.