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Respiration in Plants - Respiratory balance sheet

Grade 11CBSEBiology

Review the key concepts, formulae, and examples before starting your quiz.

🔑Concepts

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The respiratory balance sheet is a theoretical calculation of the net gain of ATPATP for every molecule of glucose oxidized during aerobic respiration.

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It is based on certain assumptions: the metabolic pathway is sequential (Glycolysis →\rightarrow TCA Cycle →\rightarrow ETS), NADHNADH synthesized in glycolysis is transferred into the mitochondria, and no intermediates of the pathway are utilized to synthesize any other compound.

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In the Electron Transport System (ETS), the oxidation of one molecule of NADHNADH gives 33 molecules of ATPATP, and one molecule of FADH2FADH_2 gives 22 molecules of ATPATP.

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During Glycolysis, there is a net gain of 22 ATPATP (Direct) and 22 NADHNADH.

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In the Link Reaction (oxidative decarboxylation of Pyruvate), 22 molecules of NADHNADH are produced per glucose molecule.

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In the Krebs Cycle (TCA), per glucose molecule (two turns), there is a gain of 22 GTPGTP (equivalent to ATPATP), 66 NADHNADH, and 22 FADH2FADH_2.

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The total theoretical yield of ATPATP per glucose molecule is 3838 ATPATP, though in most eukaryotic cells, the net gain is 3636 ATPATP due to the cost of transporting NADHNADH into the mitochondria.

📐Formulae

1NADH→3ATP1 NADH \rightarrow 3 ATP

1FADH2→2ATP1 FADH_2 \rightarrow 2 ATP

C6H12O6+6O2+38ADP+38Pi→6CO2+6H2O+38ATPC_6H_{12}O_6 + 6O_2 + 38ADP + 38P_i \rightarrow 6CO_2 + 6H_2O + 38ATP

2 (Glycolysis - Net Direct ATP)6 (Glycolysis - 2 NADH)6 (Link Reaction - 2 NADH)2 (Krebs Cycle - 2 GTP/ATP)18 (Krebs Cycle - 6 NADH)+4 (Krebs Cycle - 2 FADH2)38 (Total ATP Yield)\begin{array}{r} 2 \text{ (Glycolysis - Net Direct ATP)} \\ 6 \text{ (Glycolysis - 2 NADH)} \\ 6 \text{ (Link Reaction - 2 NADH)} \\ 2 \text{ (Krebs Cycle - 2 GTP/ATP)} \\ 18 \text{ (Krebs Cycle - 6 NADH)} \\ + 4 \text{ (Krebs Cycle - 2 FADH}_2) \\ \hline 38 \text{ (Total ATP Yield)} \end{array}

💡Examples

Problem 1:

Calculate the total number of ATPATP molecules produced specifically through substrate-level phosphorylation (SLP) during the complete oxidation of one molecule of glucose.

Solution:

The total is 44 ATPATP.

Explanation:

Substrate-level phosphorylation occurs in two stages: 22 ATPATP are produced during Glycolysis and 22 GTPGTP (which is equivalent to ATPATP) are produced during the Krebs cycle (one per turn, and there are two turns per glucose). Thus, 2 (Glycolysis)+2 (Krebs Cycle)4 ATP\begin{array}{r} 2 \text{ (Glycolysis)} \\ + 2 \text{ (Krebs Cycle)} \\ \hline 4 \text{ ATP} \end{array}

Problem 2:

How many ATPATP molecules are generated from the NADHNADH and FADH2FADH_2 produced during only the Krebs Cycle for one molecule of Glucose?

Solution:

The total is 2222 ATPATP.

Explanation:

In the Krebs cycle, one glucose molecule results in 66 NADHNADH and 22 FADH2FADH_2. Using the conversion factors: 6×3=186 \times 3 = 18 ATPATP (from NADHNADH) and 2×2=42 \times 2 = 4 ATPATP (from FADH2FADH_2). Total is 18+4=2218 + 4 = 22 ATPATP.