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Animal Physiology (AHL) - Antibody production and vaccination

Grade 12IBBiology

Review the key concepts, formulae, and examples before starting your quiz.

🔑Concepts

Antigens are foreign substances that stimulate an immune response. They are often proteins or very large polysaccharides found on the surface of pathogens, such as the glycoproteins that determine the ABOABO blood groups.

The immune response involves a process of challenge and response. Antigens are ingested by macrophages and presented to TT helper cells (THT_H cells).

Activated THT_H cells bind to specific BB lymphocytes that have the corresponding surface antibody. This interaction, mediated by cytokines, triggers clonal selection.

Clonal expansion occurs when the activated BB cell undergoes mitosis to produce a large population of plasma cells and memory cells.

Plasma cells are specialized for the synthesis and secretion of antibodies (immunoglobulinsimmunoglobulins). These molecules are YY-shaped and consist of two heavy and two light polypeptide chains.

Memory cells provide long-term immunity. If the same pathogen enters the body again, memory cells rapidly divide into plasma cells, producing a faster and greater quantity of antibodies (secondarysecondary immuneimmune responseresponse).

Vaccination involves introducing a weakened or killed form of a pathogen (or its antigens) to stimulate the production of memory cells without causing the disease.

Monoclonal antibodies are highly specific, purified antibodies produced by a hybridomahybridoma cell (a fusion of a BB lymphocyte and a myeloma/cancer cell).

Allergies result from an overactive immune response where IgEIgE antibodies bind to mast cells, causing the release of histaminehistamine when an allergen is encountered.

Zoonosis is the transmission of pathogens from animals to humans, such as the RabiesRabies virus or MERSM_ERS.

📐Formulae

B cell+Myeloma cellHybridoma cell\text{B cell} + \text{Myeloma cell} \rightarrow \text{Hybridoma cell}

Total Antibody Count=Primary Response+Secondary Response\text{Total Antibody Count} = \text{Primary Response} + \text{Secondary Response}

Structure of IgG=2×L (light chains)+2×H (heavy chains)\text{Structure of IgG} = 2 \times L \text{ (light chains)} + 2 \times H \text{ (heavy chains)}

💡Examples

Problem 1:

Calculate the probability of a transfusion reaction if an individual with blood type OO receives a transfusion of blood type ABAB.

Solution:

P=100%P = 100\% (Agglutination will occur).

Explanation:

Individuals with blood type OO have both anti-AA and anti-BB antibodies in their plasma. Since type ABAB blood cells express both AA and BB antigens, the recipient's antibodies will attack the donor cells, leading to agglutination and potential haemolysishaemolysis.

Problem 2:

Explain the role of monoclonal antibodies in a pregnancy test strip.

Solution:

The test uses antibodies specific to the hormone hCGhCG (human chorionic gonadotropin).

Explanation:

The strip contains mobile monoclonal antibodies conjugated to a dye (EnzymeEnzyme-linked). If hCGhCG is present in the urine, it binds to these antibodies. As the liquid moves up the strip, the hCGhCG-antibody complex binds to fixed antibodies in the 'test' zone, concentrating the dye and producing a visible line.

Antibody production and vaccination - Revision Notes & Key Diagrams | IB Grade 12 Biology