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Genetics - DNA Structure and Function

Grade 9IB

Review the key concepts, formulae, and examples before starting your quiz.

🔑Concepts

DNA stands for Deoxyribonucleic Acid and is the molecule that carries genetic information for the development and functioning of an organism.

The basic building block of DNA is the nucleotide, which consists of three parts: a phosphate group, a deoxyribose sugar (C5H10O4C_5H_{10}O_4), and one of four nitrogenous bases.

There are four nitrogenous bases categorized into Purines (AA and GG) and Pyrimidines (CC and TT).

Complementary Base Pairing: Adenine (AA) always pairs with Thymine (TT) via 22 hydrogen bonds, and Cytosine (CC) always pairs with Guanine (GG) via 33 hydrogen bonds.

The DNA molecule is a double helix consisting of two antiparallel strands, meaning they run in opposite directions (55' to 33' and 33' to 55').

A gene is a specific sequence of nucleotides in DNA that codes for a functional product, usually a protein. The sequence of bases determines the sequence of amino acids.

DNA is organized into structures called chromosomes. Humans typically have 2323 pairs of chromosomes (2n=462n = 46).

📐Formulae

%A=%T\%A = \%T

%G=%C\%G = \%C

%A+%T+%G+%C=100%\%A + \%T + \%G + \%C = 100\%

Number of Purines=Number of Pyrimidines\text{Number of Purines} = \text{Number of Pyrimidines}

💡Examples

Problem 1:

A sample of double-stranded DNA is analyzed and found to contain 22%22\% Cytosine (CC). Calculate the percentage of Adenine (AA) in this DNA sample.

Solution:

If C=22%C = 22\%, then by Chargaff's rule, G=22%G = 22\%. Together, C+G=44%C + G = 44\%. The remaining 56%56\% (100%44%100\% - 44\%) must be A+TA + T. Since A=TA = T, the percentage of Adenine is 56%÷2=28%56\% \div 2 = 28\%.

Explanation:

According to Chargaff's rules, in any double-stranded DNA, the amount of Guanine equals Cytosine, and the amount of Adenine equals Thymine.

Problem 2:

Given the following DNA template strand sequence: 5ATGCCGAT35'-A-T-G-C-C-G-A-T-3', determine the sequence of the complementary DNA strand.

Solution:

3TACGGCTA53'-T-A-C-G-G-C-T-A-5'

Explanation:

To find the complementary strand, you must apply base-pairing rules (ATA \leftrightarrow T and GCG \leftrightarrow C) and ensure the orientation is antiparallel (55' pairs with 33').

DNA Structure and Function - Revision Notes & Key Formulas | IB Grade 9 Science