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Biotechnology: Principles and Processes

Each subtopic includes About section, revision page link, 10 preview questions, and practice CTAs.

Principles of Biotechnology

Subtopic

Principles of Biotechnology under Biotechnology: Principles and Processes for Grade 12 CBSE.

About Topic & Revision

Preview questions (no answers)

  1. 1.

    Which of the following is the correct temperature for the 'Annealing' step in a typical PCR cycle?

    A.

    94∘C94^{\circ}C

    B.

    40−65∘C40-65^{\circ}C

    C.

    72∘C72^{\circ}C

    D.

    100∘C100^{\circ}C

  2. 2.

    In the term pBR322pBR322, what does the letter 'p' represent?

    A.

    Polymerase

    B.

    Plasmid

    C.

    Primer

    D.

    Pathogen

  3. 3.

    The processes of separation and purification of a biosynthesized product before marketing are collectively called:

    A.

    Upstream processing

    B.

    Downstream processing

    C.

    Transformation

    D.

    PCR

Download the worksheet for Biotechnology: Principles and Processes - Principles of Biotechnology to practice offline. It includes additional chapter-level practice questions.

Tools of Recombinant DNA Technology

Subtopic

Tools of Recombinant DNA Technology under Biotechnology: Principles and Processes for Grade 12 CBSE.

About Topic & Revision

Preview questions (no answers)

  1. 1.

    The ability of a host cell to take up foreign DNA is specifically termed as:

    A.

    Capacity

    B.

    Competence

    C.

    Virulence

    D.

    Efficiency

  2. 2.

    Which of the following enzymes breaks the phosphodiester bonds of the DNA backbone?

    A.

    DNA Ligase

    B.

    Restriction Endonuclease

    C.

    DNA Polymerase

    D.

    Primase

  3. 3.

    In a DNA sequence, if the sequence 5′−GAATTC−3′5'-GAATTC-3' is present on one strand, what will be the sequence on the complementary strand in the 5′→3′5' \rightarrow 3' direction?

    A.

    5′−CTTAAG−3′5'-CTTAAG-3'

    B.

    5′−GAATTC−3′5'-GAATTC-3'

    C.

    5′−TTAACG−3′5'-TTAACG-3'

    D.

    5′−GAAUUC−3′5'-GAAUUC-3'

Download the worksheet for Biotechnology: Principles and Processes - Tools of Recombinant DNA Technology to practice offline. It includes additional chapter-level practice questions.

Restriction Enzymes and Vectors

Subtopic

Restriction Enzymes and Vectors under Biotechnology: Principles and Processes for Grade 12 CBSE.

About Topic & Revision

Preview questions (no answers)

  1. 1.

    The European Federation of Biotechnology (EFB) definition of biotechnology encompasses:

    A.

    Only traditional views

    B.

    Only modern molecular biotechnology

    C.

    Both traditional views and modern molecular biotechnology

    D.

    Only plant tissue culture

  2. 2.

    Which of the following enzymes is used to break the cell wall of fungi during DNA isolation?

    A.

    Cellulase

    B.

    Lysozyme

    C.

    Chitinase

    D.

    Amylase

  3. 3.

    What is the significance of using a single recognition site for a restriction enzyme in a vector?

    A.

    It makes the vector unstable

    B.

    It simplifies the cloning process by preventing fragmentation of the vector

    C.

    It allows for faster replication

    D.

    It helps in selecting only large genes

Download the worksheet for Biotechnology: Principles and Processes - Restriction Enzymes and Vectors to practice offline. It includes additional chapter-level practice questions.

Processes of Recombinant DNA Technology (PCR, Bioreactors)

Subtopic

Processes of Recombinant DNA Technology (PCR, Bioreactors) under Biotechnology: Principles and Processes for Grade 12 CBSE.

About Topic & Revision

Preview questions (no answers)

  1. 1.

    PCR is a technique used for the amplification of:

    A.

    Proteins

    B.

    Lipids

    C.

    DNA

    D.

    Carbohydrates

  2. 2.

    Which of these is a heat-stable DNA polymerase?

    A.

    DNA Polymerase I

    B.

    DNA Polymerase III

    C.

    Taq polymerase

    D.

    RNA Polymerase

  3. 3.

    In a continuous culture system, fresh medium is added from one side while the used medium is drained from the other side to:

    A.

    Stop the growth

    B.

    Keep the cells in stationary phase

    C.

    Keep the cells in their physiologically most active log phase

    D.

    Decrease the product yield

Download the worksheet for Biotechnology: Principles and Processes - Processes of Recombinant DNA Technology (PCR, Bioreactors) to practice offline. It includes additional chapter-level practice questions.