Biotechnology: Principles and Processes
Each subtopic includes About section, revision page link, 10 preview questions, and practice CTAs.
Principles of Biotechnology
SubtopicPrinciples of Biotechnology under Biotechnology: Principles and Processes for Grade 12 CBSE.
Preview questions (no answers)
- 1.
Which of the following is known as 'molecular scissors' in biotechnology?
A.DNA Ligase
B.DNA Polymerase
C.Restriction Endonucleases
D.Helicases
- 2.
Who among the following was responsible for constructing the first recombinant DNA molecule in 1972?
A.Watson and Crick
B.Arber and Nathans
C.Stanley Cohen and Herbert Boyer
D.Mullis and Smith
- 3.
In the nomenclature of the restriction enzyme , what does the Roman numeral 'I' indicate?
A.The first letter of the species name
B.The order in which the enzyme was isolated from the strain of bacteria
C.The strain of the bacteria used
D.The genus name of the bacteria
Download the worksheet for Biotechnology: Principles and Processes - Principles of Biotechnology to practice offline. It includes additional chapter-level practice questions.
Tools of Recombinant DNA Technology
SubtopicTools of Recombinant DNA Technology under Biotechnology: Principles and Processes for Grade 12 CBSE.
Preview questions (no answers)
- 1.
Which enzyme is used to join the sticky ends of a DNA fragment and a vector DNA?
A.DNA Polymerase
B.RNA Polymerase
C.Restriction Endonuclease
D.DNA Ligase
- 2.
The specific DNA sequence recognized by a restriction endonuclease to bind and cut the DNA is called a:
A.TATA box sequence
B.Palindromic nucleotide sequence
C.Polyadenylation signal
D.Promoter sequence
- 3.
In the nomenclature of restriction enzymes like , what does the Roman numeral 'I' indicate?
A.The order in which the enzyme was isolated from the strain of bacteria
B.The number of times the enzyme cuts the DNA
C.The number of subunits present in the enzyme
D.The priority of the enzyme in the laboratory protocol
Download the worksheet for Biotechnology: Principles and Processes - Tools of Recombinant DNA Technology to practice offline. It includes additional chapter-level practice questions.
Restriction Enzymes and Vectors
SubtopicRestriction Enzymes and Vectors under Biotechnology: Principles and Processes for Grade 12 CBSE.
Preview questions (no answers)
- 1.
Which vector is popularly used to deliver genes into dicot plants by utilizing its natural 'disarming' ability?
A.pBR322
B.Disarmed Agrobacterium tumefaciens
C.Bacteriophage Lambda
D.pUC18
- 2.
Which of the following is a key feature of a DNA palindromic sequence recognized by restriction enzymes?
A.The sequence is read the same on the two strands when orientation is kept the same ()
B.The sequence contains only Adenine and Guanine
C.The sequence is read differently in direction
D.The sequence is identical on the same strand in both directions
- 3.
In the nomenclature of restriction enzymes like , what does the Roman numeral 'I' indicate?
A.The order in which the enzyme was isolated from the strain of bacteria
B.The genus to which the bacteria belongs
C.The specific strain of the bacteria used
D.The number of recognition sites present on the DNA
Download the worksheet for Biotechnology: Principles and Processes - Restriction Enzymes and Vectors to practice offline. It includes additional chapter-level practice questions.
Processes of Recombinant DNA Technology (PCR, Bioreactors)
SubtopicProcesses of Recombinant DNA Technology (PCR, Bioreactors) under Biotechnology: Principles and Processes for Grade 12 CBSE.
Preview questions (no answers)
- 1.
Which of the following processes collectively refers to the separation and purification of a finished product before it is marketed?
A.Upstream processing
B.Downstream processing
C.Elution
D.Transformation
- 2.
What is the primary purpose of the 'sparger' in a stirred-tank bioreactor?
A.To maintain constant temperature
B.To monitor the pH of the culture
C.To ensure even mixing of nutrients
D.To bubble air and increase oxygen availability
- 3.
The enzyme polymerase used in PCR is isolated from which of the following organisms?
A.Agrobacterium tumefaciens
B.Escherichia coli
C.Thermus aquaticus
D.Bacillus thuringiensis
Download the worksheet for Biotechnology: Principles and Processes - Processes of Recombinant DNA Technology (PCR, Bioreactors) to practice offline. It includes additional chapter-level practice questions.