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Biological Molecules - Structure of DNA

Grade 11IGCSEBiology

Review the key concepts, formulae, and examples before starting your quiz.

🔑Concepts

DNADNA (Deoxyribonucleic acid) is the molecule that contains the genetic instructions for the development, functioning, growth, and reproduction of all known organisms.

The basic unit of DNADNA is a nucleotide, which consists of three parts: a phosphate group, a pentose sugar (deoxyribose), and a nitrogenous base.

There are four types of nitrogenous bases in DNADNA: Adenine (AA), Thymine (TT), Cytosine (CC), and Guanine (GG).

The DNADNA molecule consists of two strands coiled together to form a double helix.

The two strands are held together by hydrogen bonds between complementary base pairs: AA always pairs with TT, and CC always pairs with GG.

The 'backbone' of the DNADNA strand is formed by alternating sugar and phosphate groups, linked by strong covalent bonds.

The sequence of bases along a DNADNA molecule forms the genetic code, which determines the specific proteins synthesized by the cell.

📐Formulae

[A]=[T][A] = [T]

[C]=[G][C] = [G]

[A]+[G]=[T]+[C]=50%[A] + [G] = [T] + [C] = 50\%

Total percentage=[A]+[T]+[C]+[G]=100%\text{Total percentage} = [A] + [T] + [C] + [G] = 100\%

Phosphate Group=PO43\text{Phosphate Group} = PO_4^{3-}

💡Examples

Problem 1:

A sample of double-stranded DNADNA is analyzed and found to contain 22%22\% Cytosine (CC). Calculate the percentage of Adenine (AA) present in the sample.

Solution:

28%28\%

Explanation:

According to Chargaff's rules, the amount of Cytosine is equal to Guanine, so [C]=[G]=22%[C] = [G] = 22\%. The total percentage of C+G=22%+22%=44%C + G = 22\% + 22\% = 44\%. The remaining percentage for A+TA + T is 100%44%=56%100\% - 44\% = 56\%. Since [A]=[T][A] = [T], the percentage of Adenine is 56%÷2=28%56\% \div 2 = 28\%.

Problem 2:

Identify the number of hydrogen bonds formed between a sequence of three base pairs: ATA-T, GCG-C, and CGC-G.

Solution:

88 hydrogen bonds

Explanation:

In DNADNA base pairing, an ATA-T pair is held together by 22 hydrogen bonds, while a GCG-C (or CGC-G) pair is held together by 33 hydrogen bonds. Therefore, the total is 2+3+3=82 + 3 + 3 = 8 bonds.

Structure of DNA - Revision Notes & Key Diagrams | IGCSE Grade 11 Biology